Burnett Lab

Cellular Proteolysis and Neurodegeneration

Protein Homeostasis
Identifying the genes and pathways that regulate protein degradation

The Ubiquitin Proteasome System
Cytoplasmic Proteases
 Lysosomes break down  fats, carbohydrates, proteins, and other macromolecules into simple compounds. Lysosomes utilize approximately 40 different  hydrolytic enzymes, all of which are manufactured in the endoplasmic reticulum and modified in the Golgi apparatus. 
Proteases represent one of the largest families of enzymes and are involved in almost every aspect of cellular function. All proteases catalyze the hydrolysis of amide (and ester) bonds using a reaction involving nucleophilic attack on the carbonyl carbon of the scissile bond. 
The ubiquitin proteasome system (UPS) plays a major role in maintaining cellular homeostasis. Dysregulation of the UPS is associated with a number of neuropathological abnormalities including neurodegenerative diseases, ischemic brain damage, and cognitive disorders.